Changes in the firing properties of PMNsa
E16 | E18 | P0–P1 | |
---|---|---|---|
Firing threshold,I thr, pAa | 41 ± 5 | 48 ± 13 | 98 ± 18* ,‡ |
Firing frequency, Hzb | |||
I thr | 7.1 ± 0.5 | 9.8 ± 0.9 | 13.2 ± 0.7* ,‡ |
2 ×I thr | 14.4 ± 1.0 | 18.4 ± 1.3† | 28.0 ± 2.3* ,‡ |
First ISI frequency, Hz | |||
I thr | 12.6 ± 0.9 | 13.9 ± 1.8 | 29.6 ± 3.7* ,‡ |
2 × I thr | 24.1 ± 2.2 | 27.9 ± 2.6 | 52.5 ± 5.3* ,‡ |
Current–frequency slope,cHz/nA | |||
First ISI | 444 ± 51 | 600 ± 75 | 501 ± 60 |
Last ISI | 340 ± 43 | 327 ± 41 | 271 ± 44 |
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Results were compiled from the firing discharges of PMNs that illustrate repetitive firing patterns similar to those in Fig. 8, A–C (i.e., bursting and high-threshold PMNs not included in data), after a series of 1-s depolarizing current pulses of increasing amplitude from a holding potential of approximately −60 mV. Measurements of the interspike intervals (ISIs) were carried out at the beginning and the end of the depolarizing pulse. a Firing threshold was determined as the lowest stimulation current necessary to evoke repetitive firing. b Firing frequency was determined as the number of action potentials per 1-s stimulation.c Current–frequency slope was the ratio of the first or last ISI frequency vs. injected current. (*) P ≤ 0.01, (†)P ≤ 0.05 vs. E16 or (‡)P≤ 0.05 vs. E18.