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Article: M1 receptors mediate cholinergic modulation of excitability in neocortical pyramidal neurons.

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Gulledge AT; Bucci DJ; Zhang SS; Matsui M; Yeh HH
J. Neurosci., 2009


Table 1.

Comparison of physiological properties and phasic cholinergic responsiveness in pyramidal neurons from different cortical layers and genetic backgrounds

Genotype Layer n RMP (mV) RN (MΩ) Sag (%) “SK” response (mV) Spike acceleration (%)
Wild type 2/3 15 −85 ± 3† 140 ± 30† 3 ± 1† +0.4 ± 0.5† +34 ± 22†
M1 KO 2/3 8 −84 ± 2 185 ± 30* 3 ± 1 +0.3 ± 0.5 −10 ± 13*
M3/M5 KO 2/3 16 −85 ± 3 154 ± 49 4 ± 3 +0.6 ± 1.0 +22 ± 13
Wild type 5 15 −80 ± 2 75 ± 33 15 ± 3 −2.3 ± 0.8 +75 ± 43
M1 KO 5 14 −81 ± 2 89 ± 53 14 ± 5 +0.1 ± 0.2* −1 ± 8*
M3 KO 5 11 −80 ± 3 68 ± 30 17 ± 5 −2.1 ± 1.1 +52 ± 47
M5 KO 5 7 −81 ± 2 99 ± 41 10 ± 4 −2.4 ± 1.2 +43 ± 40
M3/M5 KO 5 7 −81 ± 2 82 ± 23 10 ± 5 −2.4 ± 1.3 +59 ± 17

  • Comparison of physiological characteristics and phasic cholinergic responses at resting membrane potentials (SK response) or during current-evoked action potential firing (spike acceleration). Numbers in bold indicate p < 0.05 for cholinergic-induced ″SK″ responses and spike acceleration (paired t test).

  • ↵*p < 0.01 compared with other groups of cells in the same cortical layer (Kruskal–Wallis test).

  • ↵†p < 0.01 when wild-type layer 2/3 neurons are compared with wild-type layer 5 neurons (Mann–Whitney test). KO, Genetic knock-out for indicated mAChR subtype; Sag, percent voltage rectification at steady-state relative to peak potential reached during negative current injections.


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Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source
Neocortex pyramidal cell layer 5-6 medial prefrontal cortex layer 5 pyramidal cell resting membrane potential (mV) -80.0 ± 2.0 (15) -80.0 (mV) Data Table
Neocortex pyramidal cell layer 5-6 medial prefrontal cortex layer 5 pyramidal cell input resistance (MΩ) 75.0 ± 33.0 (15) 75.0 (MΩ) Data Table
Neocortex pyramidal cell layer 5-6 medial prefrontal cortex layer 5 pyramidal cell sag ratio 15.0 ± 3.0 (15) 0.15 (ratio) Data Table
Neocortex pyramidal cell layer 2-3 medial prefrontal cortex layer 2-3 pyramidal cell resting membrane potential (mV) -85.0 ± 3.0 (15) -85.0 (mV) Data Table
Neocortex pyramidal cell layer 2-3 medial prefrontal cortex layer 2-3 pyramidal cell input resistance (MΩ) 140.0 ± 30.0 (15) 140.0 (MΩ) Data Table
Neocortex pyramidal cell layer 2-3 medial prefrontal cortex layer 2-3 pyramidal cell sag ratio 3.0 ± 1.0 (15) 0.03 (ratio) Data Table