AboutNeuron TypesElectrophysiology PropertiesArticlesFAQsData/APIGet Involved

Article: Submillisecond optical reporting of membrane potential in situ using a neuronal tracer dye.

Full Text (publisher's website) ; Article Metadata ; Article Data (extracted)
Bradley J; Luo R; Otis TS; DiGregorio DA
J. Neurosci., 2009


Table 1.

Summary of DPA effects on Purkinje neuron excitability

Control 1 μm DPA 2 μm DPA 5 μm DPA
Properties of single APs
    Number of cells 10 5 10 7
    AP height (mV) 79.3 ± 1.5 74.9 ± 6.6 77.3 ± 3 76.8 ± 2.9
    AP half-width (μs) 431 ± 29 422 ± 42 422 ± 24 637 ± 36**
    AP threshold (mV) −54.9 ± 1.2 −52.3 ± 2.4 −53.6 ± 1 −52.6 ± 2.7
    Threshold stimulus (nA) 1.3 ± 0.17 1.2 ± 0.13 1.3 ± 0.11 1.1 ± 0.23
Properties of complex spike-like bursts
    Number of cells 7 6 6 7
    No. of spikelets per burst 3.7 ± 0.2 3.7 ± 0.2 3.3 ± 0.2 2.3 ± 0.2*
    Intraburst freq. (Hz) 362 ± 19 334 ± 17 329 ± 17 219 ± 29*

  • Single APs or complex spike-like bursts were elicited in Purkinje neurons by current injection in whole-cell current-clamp mode under control conditions, or after incubating the slice in the indicated concentration of DPA for greater than 45 min. AP half-width was measured at the half-maximal amplitude of the AP. AP threshold was measured by calculating the membrane potential at the time point corresponding to the minimum of the differentiated membrane potential versus time. The threshold stimulus is defined as the minimal current necessary to elicit an AP within 1.3–1.4 ms after the current step onset. Number of spikelets per burst and intraburst frequency were measured from complex spike-like bursts.

  • ↵**Significant difference of the 5 μm group from all others at p ≤ 0.005;

  • ↵*significant difference of the 5 μm group from control at p < 0.005 based on a one-way ANOVA with a post hoc Kruskal–Wallis test.


Report miscurated data

Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source
Cerebellum Purkinje cell spike amplitude (mV) 79.3 ± 1.5 (10) 79.3 (mV) Data Table
Cerebellum Purkinje cell spike half-width (μs) 431.0 ± 29.0 (10) 0.431 (ms) Data Table
Cerebellum Purkinje cell spike threshold (mV) -54.9 ± 1.2 (10) -54.9 (mV) Data Table
Cerebellum Purkinje cell rheobase (nA) 1.3 ± 0.17 (10) 1300.0 (pA) Data Table